Temporary

Part:BBa_M11007:Design

Designed by: Gerald McEwen   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-15)

NADH-dependent butanol dehydrogenase A (bdhA/BDH I)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 504


Design Notes

bdhA comes from the (-) strand, bdhA follows bdhB in the actual sequence from Clostridium acetobutylicum ATCC 824

Source

bdhA is from Clostridium acetobutylicum ATCC 824: NC_003030 on the (-)strand

References

[1] "Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes." Walter K.A., Bennett G.N., Papoutsakis E.T. J. Bacteriol. 174:7149-7158(1992) [PubMed: 1385386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

[2] "Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

[3] "Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium acetobutylicum." Petersen D.J., Welch R.W., Walter K.A., Mermelstein L.D., Papoutsakis E.T., Rudolph F.B., Bennett G.N. Ann. N. Y. Acad. Sci. 646:94-98(1991) [PubMed: 1809209] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS.