Composite

Part:BBa_M10216

Designed by: Jennifer Brophy   Group: Berkeley BioE140L - S09   (2009-05-10)

{Pbad.rbs.prepro.StrepTag}{AG4}{CPompX!}{dblTerm}

Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a silver binding peptide {AG4}, circularly permutated ompX {CPompX!} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}


Streptavidin binding assay template
Growth curve assay template
Silver binding assay template


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1679
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
    Illegal PstI site found at 1679
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1679
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1679
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005


[edit]
Categories
Parameters
None