Coding
Part:BBa_M10086:Design
Designed by: Jennifer Brophy Group: Berkeley BioE140L - S09 (2009-05-01)
{˂CPG_L2!}
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 253
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Needed to design appropriate linkers (to fuse the N and C termini of the native protien). Used 6aa linker (CDS: GGATCTAAGTCTCGTCGC) from PMID: 18480093.
The opening was created in loop 2 (aa 59-60) because the residues of the mature protein are presumably disordered because they are not visible in electron density maps.
Source
E. coli MG1655 genomic DNA