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Part:BBa_M10021:Design

Designed by: Nikhil Sharma   Group: Berkeley BioE140L - S09   (2009-05-10)


{˂Poly_bind˃} passenger protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-Removed prepro sequences



Source

Made from PCR assembly from synthetic sources.

Construction of Polystyrene-Binding Peptide

Wobble Ons003F/Ons004R (67bp, EcoRI/BamHI)

Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)

Product is pBca9495AK-M10021 {<Poly_bind>}

Ons003F Forward construction of Poly_bind basic part ccataGAATTCatgAGATCTttcaaattctggctatacgaacatg

Ons004R Reverse construction of Poly_bind basic part ctgatGGATCCcccccgtattacatgttcgtatagccagaat


References