DNA
Part:BBa_M1000
Designed by: Tom Knight Group: UtahState_BE5930_-_S11 (2006-03-16)
Left mosaic end for Tn5 transpososome
- Left end of a linear transposome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII cut sites, or be PCR-amplified with blunt-end-producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG.
- This part, and the companion part M1001 provide BioBrick tools to construct Tn5 transposomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 transposase protein, electroportated into cells, and finally randomly insert into the bacterial chromosome.
- The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//DNA/transposome/Tn5
//chassis/prokaryote/mflorum
//chassis/prokaryote/mflorum
None |