Translational_Unit
Part:BBa_K993003:Design
Designed by: FURKAN SACİT CEYLAN Group: iGEM13_AUC_TURKEY (2013-06-14)
Urease generator with ROSE thermometer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 679
Illegal NgoMIV site found at 874
Illegal NgoMIV site found at 1702 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 742
Design Notes
Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.