Translational_Unit

Part:BBa_K993003:Design

Designed by: FURKAN SACİT CEYLAN   Group: iGEM13_AUC_TURKEY   (2013-06-14)


Urease generator with ROSE thermometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 679
    Illegal NgoMIV site found at 874
    Illegal NgoMIV site found at 1702
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 742


Design Notes

Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.


References