Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
Translational_Unit

Part:BBa_K993001:Design

Designed by: Mustafa Elitok   Group: iGEM13_AUC_TURKEY   (2013-06-07)


RFP Generator with ROSE thermometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 749
    Illegal AgeI site found at 861
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.


Source

Bradyrhizobium japonicum USDA 110 and Registry

References