Composite
Part:BBa_K988003:Design
Designed by: Isaac Ward Group: iGEM13_Lethbridge_Canada (2013-06-21)
K314100+K988002
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
Illegal NgoMIV site found at 581 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Their is a lack of inducible promoters with RBS in the registry, so an constitutive promoter was used instead to express the protein.
Source
https://parts.igem.org/Part:BBa_K314100 https://parts.igem.org/Part:BBa_K988002