Composite

Part:BBa_K988003:Design

Designed by: Isaac Ward   Group: iGEM13_Lethbridge_Canada   (2013-06-21)


K314100+K988002


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 581
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Their is a lack of inducible promoters with RBS in the registry, so an constitutive promoter was used instead to express the protein.


Source

https://parts.igem.org/Part:BBa_K314100 https://parts.igem.org/Part:BBa_K988002

References