Generator

Part:BBa_K988002:Design

Designed by: Isaac Ward   Group: iGEM13_Lethbridge_Canada   (2013-06-21)


Oxytocin-Neurophysin1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was back translated from the protein sequence to ensure the right DNA sequence was obtained for the proper protein sequence. When adding the PelB signal sequence it was was very important to consider the cleavage site as the C-C disulfide bond was necessary to be translated, without a signal sequence the protein would have to start with a Methionine, with would disrupt the C-C disulfide bond. the His tag was added to help with with protein Purification.


Source

This Part was developed from the Homosapien sapien coding sequence for Oxytocin-Neurophysin1

References