Composite

Part:BBa_K976008:Design

Designed by: Amna Sohail & Molly Petrous & Tyler Knox & Mackenzie Reetz   Group: iGEM13_MCIT_Indianapolis   (2013-06-21)


Yeast ADH1 Promoter + Firefly Luciferase + Yeast ADH1 Promoter + Renilla Luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
    Illegal NgoMIV site found at 3292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 830
    Illegal BsaI site found at 3942
    Illegal BsaI.rc site found at 5110
    Illegal SapI.rc site found at 2259


Design Notes

None.


Source

The Yeast ADH1 promoter portion was derived from the BioBrick, BBa_J63005, which was designed by Caroline Ajo-Franklin of the Silver Lab 2006 Group. The Firefly Luciferase portion was derived from the BioBrick, BBa_I712019, which was designed by Anja Korenčič of the 2007 Ljubljana Group. The Renilla Luciferase reporter is derived from the BioBrick, BBa_J52008, which was designed by Monika Ciglic of the iGEM Slovenia 2006 Group.

References