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Part:BBa_K957014:Design
Designed by: Steven Tiwen Chen & Dylan Patrick Webster Group: iGEM12_Cornell (2012-10-28)
Salicylate inducible polHis-MtrB, alternate
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Constructed using non-BioBrick AscI cutsite of BBa_K957000.
Source
mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source.
The salicylate sensitive region was isolated via PCR from a plasmid containing BBa_K228227, designed by PKU_Beijing 2009.