Intermediate

Part:BBa_K952005:Design

Designed by: Saimon Sharif   Group: iGEM12_Columbia-Cooper-NYC   (2012-10-01)


IPTG inducible promoter-B0034-YF1-B0034-FixJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 790
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 970
    Illegal NgoMIV site found at 1482
    Illegal NgoMIV site found at 1775
    Illegal NgoMIV site found at 1869
    Illegal AgeI site found at 504
    Illegal AgeI site found at 1650
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1539
    Illegal BsaI.rc site found at 403


Design Notes

N/A


Source

BBa_J04500 was obtained from the 2012 kit. BBa_K592016 is available in the parts registry, but was obtained directly from the Uppsala-Sweden 2011 team.

References