Intermediate

Part:BBa_K952004:Design

Designed by: Saimon Sharif   Group: iGEM12_Columbia-Cooper-NYC   (2012-10-01)


constitutive promoter-B0034-YF1-B0034-FixJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

N/A


Source

BBa_J23102 was obtained from the 2012 kit. BBa_K592016 is available in the parts registry, but was obtained directly from the Uppsala-Sweden 2011 team.

References