Plasmid

Part:BBa_K950010

Designed by: Jakob Matthes   Group: iGEM12_Tuebingen   (2012-09-13)

pRS315 shuttle vector

pRS315 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the LEU2 gene for selection.

Team Tuebingen 2015 decided to make this vector backbone Biobrick compatible (RFC10). You can find that part here: Part:BBa_K1680015.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal XbaI site found at 3148
    Illegal SpeI site found at 3154
    Illegal PstI site found at 3172
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal SpeI site found at 3154
    Illegal PstI site found at 3172
    Illegal NotI site found at 3140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal BamHI site found at 3160
    Illegal XhoI site found at 2410
    Illegal XhoI site found at 3211
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal XbaI site found at 3148
    Illegal SpeI site found at 3154
    Illegal PstI site found at 3172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal XbaI site found at 3148
    Illegal SpeI site found at 3154
    Illegal PstI site found at 3172
    Illegal NgoMIV site found at 2799
    Illegal AgeI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4584
    Illegal SapI site found at 3501
    Illegal SapI site found at 5605


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