Regulatory

Part:BBa_K950003:Design

Designed by: Jan Rudolph   Group: iGEM12_Tuebingen   (2012-07-29)

yeast suc2 promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

length of promoter was chosen to include binding sites and to obtain good primers

Source

yeast genome, see http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=suc2

References

  1. [http://www.ncbi.nlm.nih.gov/pubmed/9794821 CARLSON, M. 1998. Regulation of glucose utilization in yeast. Current Opinion in Genetics & Development, 8, 560-564.]
  2. [http://www.ncbi.nlm.nih.gov/pubmed/3526554 CELENZA, J. & CARLSON, M. 1986. A yeast gene that is essential for release from glucose repression encodes a protein kinase. Science, 233, 1175-1180.]
  3. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC231480/ LUTFIYYA, L. L. & JOHNSTON, M. 1996. Two zinc-finger-containing repressors are responsible for glucose repression of SUC2 expression. Mol Cell Biol, 16, 4790-7.]
  4. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC552004/ NEHLIN, J. O. & RONNE, H. 1990. Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins. Embo j, 9, 2891-8.]
  5. [http://www.ncbi.nlm.nih.gov/pubmed/9730278 WU, J. & TRUMBLY, R. J. 1998. Multiple regulatory proteins mediate repression and activation by interaction with the yeast Mig1 binding site. Yeast, 14, 985-1000.]