Coding

Part:BBa_K945007:Design

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-27)


Pichia pastoris optimized His-CrtB-PTS1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1529
    Illegal XbaI site found at 1558
    Illegal PstI site found at 1342
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1529
    Illegal PstI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1529
    Illegal BamHI site found at 1333
    Illegal BamHI site found at 1364
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1529
    Illegal XbaI site found at 1558
    Illegal PstI site found at 1342
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1529
    Illegal XbaI site found at 1558
    Illegal PstI site found at 1342
    Illegal AgeI site found at 1052
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 976
    Illegal BsaI.rc site found at 1316
    Illegal SapI site found at 14


Design Notes

The codon optimization for the sequence had to take into account the illegal restriction sites to be avoided in order to be compatible with assembly standards.


Source

This sequence is originally derived from the CDS for phytoene synthase from Pantoea ananatis.

References