Regulatory

Part:BBa_K945000:Design

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-26)


AOX1 yeast promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1016
    Illegal PstI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 47
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 53
    Illegal BglII site found at 75
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1016
    Illegal PstI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1016
    Illegal PstI site found at 47
    Illegal NgoMIV site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A high incidence of nucleotide repetitions in the sequence makes it especially difficult for the part to be manufactured by oligonucleotide synthesis.


Source

This sequence was identified from the 5' untranslated region of the alcohol oxidase gene (AOX1) present in the methylotrophic yeast Pichia pastoris.

References