Coding

Part:BBa_K944003:Design

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)


cioA gene related to cyanide insensitivity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 434
    Illegal XhoI site found at 322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 747
    Illegal NgoMIV site found at 988
    Illegal NgoMIV site found at 1129
    Illegal NgoMIV site found at 1371
    Illegal NgoMIV site found at 1393
    Illegal AgeI site found at 280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1336
    Illegal SapI.rc site found at 1411


Design Notes

This part has no restriction sites used in the protocol standard 10.


Source

This came from the Pseudomonas aeruginosa genomic sequence we searched in genbank and synthesized by IDT Technologies.

References