Coding

Part:BBa_K936034

Designed by: Sergio Castro Waqued   Group: iGEM12_UC_Davis   (2012-10-03)

LC Cutinase mutant Y127A

Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The tyrosine at the 127th amino acid position is changed to an alanine. This part was created using site directed mutagenesis to change a "TAC" codon to a "GCC" codon.
UC_Davis_First_pNPB_Run.png
(Above Graph) Results of pNPB assay detailing the constitutive construct as having the highest esterase activity.UC_Davis_Third_pNPB_Run.png
(Above Graphs) The graphs above detail higher activity among the constitutive construct and some mutant variants in esterase activity compared to the control and wild type per unit cell.
UC_Davis_Last_pNPB_Run.png
(Above graph) This graph represents a possible production of cutinase by the cells upon entering stationary phase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None