Coding
Part:BBa_K936032:Design
Designed by: Sergio Castro Waqued Group: iGEM12_UC_Davis (2012-10-03)
LC Cutinase mutant S101A
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 600
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Eliminated BioBrick restriction sites and added a TAA double stop.
Source
Modified from the paper Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach by Sulaiman et al.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294458/