Coding

Part:BBa_K936032:Design

Designed by: Sergio Castro Waqued   Group: iGEM12_UC_Davis   (2012-10-03)


LC Cutinase mutant S101A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Eliminated BioBrick restriction sites and added a TAA double stop.


Source

Modified from the paper Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach by Sulaiman et al.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294458/

References