Composite

Part:BBa_K936019:Design

Designed by: Pui Yan Ho   Group: iGEM12_UC_Davis   (2012-09-11)

R0010+B0034+Dehydrogenase+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 459
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ligated Dehydrogenase to the LacI promoter to specifically control the amount of Dehydrogenase produced, and tracking if the production level of one enzyme prevents any bottleneck buildups of an intermediate in the pathway.

Source

e. coli MG1655

References