Part:BBa_K933002:Design
short leader sequence with mCherry and GFP reporters
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 71
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 71
Illegal NheI site found at 99
Illegal NheI site found at 122
Illegal NotI site found at 77 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 71
Illegal BamHI site found at 202 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 71
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 71
Illegal XbaI site found at 86 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
An alternative RBS site was used to reduce mCherry expression in this construct. There is an RBS30 site before the GFP so that the GFP will be expressed not depending on the mCherry expression. The repeat sequence that is inputted will cause the variation in RFP expression. The parts were constructed in the sPB1C3 vector as the backbone.
Only sequence provided was for: EcoR1, Xba1, J23100 Promoter, RBS34, leader sequence, BamHI, repeat sequence spot, HindIII
the rest of sequence is the mCherry and GFP
Source
This part was partially assembled by a Penn State Ph.D student in Dr. Thomas Richard's lab named Mike Speer. Completion of the codon optimization project was performed by iGEM members. An alternative RBS site was used to reduce mCherry expression.