Coding
Part:BBa_K929202:Design
Designed by: Potsdam Bioware 2012 iGEM Group: iGEM12_Potsdam_Bioware (2012-09-24)
EGF-Receptor_3rd domain with PelB leadersequence, His-tag and C-terminal Sortase motif
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 894
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 664
Illegal AgeI site found at 1342 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
The Biobrickpart was designed with restriction sites according to the RFC25 suffix and prefix. We cloned the desired part into the Biobrick containing CMV promoter (BBa_I712004). The resulting Biobrick (BBa_K929202) corresponds to the original RFC10 prefix and suffix. But the desired fusionparts PelB, EGF-Receptor_3rd domain, His-tag and C-terminal sortase motif have 2 additional enclosing restriction sites according to the RFC25. That means, that the Part 'PelB, EGF-Receptor_3rd domain, His-tag and C-terminal sortase motif' could be cut out with the enzyms NgoMIV and AgeI
References
- Leung K. Cy5.5-Ac-Cys-ZEGFR:1907. 2012 Jun 21 [updated 2012 Sep 07]. Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda(MD): National Center for Biotechnology Information (US); 2004-2012. [http://www.ncbi.nlm.nih.gov/pubmed/22973578 PubMed PMID: 22973578]
- Zhixiang Wang (2012). Mutual Regulation of Receptor-Mediated Cell Signalling and Endocytosis: EGF Receptor System as an Example, Molecular Regulation of Endocytosis, Brian Ceresa (Ed.), ISBN: 978-953-51-0662-3, InTech, [http://www.intechopen.com/books/molecular-regulation-of-endocytosis/mutual-regulation-of-receptor-mediated-cell-signalling-and-endocytosis-egf-receptor-system-as-an-exa InTech]
- Abdullah SE, Haigentz M Jr, Piperdi B. Dermatologic Toxicities from Monoclonal Antibodies and Tyrosine Kinase Inhibitors against EGFR: Pathophysiology and Management. Chemother Res Pract. 2012;2012:351210. Epub 2012 Sep 11. PubMed PMID: 22997576; [http://www.ncbi.nlm.nih.gov/pubmed/22997576 PMCID: PMC3446637]
- Ayako Kamei, Paul S. Hauser, Jennifer A. Beckstead, Paul M.M. Weers, Robert O. Ryan Expressed protein ligation using an N-terminal cysteine containing fragment generated in vivo from a pelB fusion protein. Published in final edited form as: Protein Expr Purif. 2012 June; 83(2): 113–116. Published online 2012 April 1. doi: 10.1016/j.pep.2012.03.014 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1963442/ PMCID: PMC1963442]