![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K929201:Design
Designed by: Potsdam Bioware 2012 iGEM Group: iGEM12_Potsdam_Bioware (2012-09-24)
N-terminal sortase motif linked to AAV2-VP2 with kozak and CMV
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2030
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2666
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2589
Illegal SapI site found at 1467
Design Notes
Primer (forward) with XbaI restriction site, kozak consensus sequence, Sortase motif:
Primer (reverse, complement) with PstI restriction site:
Proceeding
Source
pCMV_DARPin-E01_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) (BBa_K404161) and self-designed primers