Part:BBa_K929106
B-cell transmembrane region with mCherry
General information
| B-cell transmembrane region with mCherry | |
|---|---|
| |
| BioBrick Nr. | BBa_929106 |
| RFC standard | RFC 25 |
| Requirement | pSB1C3 |
| Source | Gene synthesis by GeneArt |
| Submitted by | [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012] |
This part is a composite part of the transmembrane domain of a B-cell receptor with a 3 aa linker fused to the mcherry reporter.
Transmembrane domain of B-cell receptor with glycine-serine linker:
Helical single-span transmembrane domain of the B-Cell-Receptor with a flexible 3 aa linker at the C-terminal end. This part was designed for fusion to proteins or peptides that will be presented on the cells surface. A signal peptide at the N-terminal end of the fusion protein for membrane integration (e.g. part BBa_K157001) is highly required.
mCherry:
Expression of the fusion part and its cellular localization can be monitored by the mcherry signal with an excitation at 587 nm and an emission at 610 nm.
Characterization
For characterization and further applications see BBa_K929107.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |