Coding

Part:BBa_K925007:Design

Designed by: Michael Paul Lockhart   Group: iGEM12_St_Andrews   (2012-09-24)


MyToxic


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 195
    Illegal PstI site found at 414
    Illegal PstI site found at 637
    Illegal PstI site found at 856
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 195
    Illegal PstI site found at 414
    Illegal PstI site found at 637
    Illegal PstI site found at 856
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 105
    Illegal BamHI site found at 547
    Illegal XhoI site found at 441
    Illegal XhoI site found at 883
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 195
    Illegal PstI site found at 414
    Illegal PstI site found at 637
    Illegal PstI site found at 856
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 195
    Illegal PstI site found at 414
    Illegal PstI site found at 637
    Illegal PstI site found at 856
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part had to be designed so as the metal binding sites would not interefere with each others function.


Source

This part is biologically synthesised.

References