Coding

Part:BBa_K925004:Design

Designed by: Michael Paul Lockhart   Group: iGEM12_St_Andrews   (2012-09-24)


NiTagGST


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 292
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 292
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the design of this part our main consideration was the difficulty of inserting a very small peice of DNA into the pGEX-6P-1 plasmid. This created difficulty as we could not do diagnostic digests and our vector was more likely to self-ligate.


Source

This part was biologically synthesised.

References