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Part:BBa_K917001:Experience

Designed by: Chris French   Group: iGEM12_Edinburgh   (2012-06-29)

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Applications of BBa_K917001

Expression of this BioBrick allows growth of E. coli K12-derived strains with sucrose as sole carbon source in presence of sodium arsenate. We (Edinburgh 2012) have tested this BioBrick by growing transformed E. coli on range of different media; Results below were obtained after 4 days of incubation at 37 degrees Celcius. Transformed cells managed to from colonies on plate with sucrose added as sole carbon source, whereas vector control cells failed to grow.

  • Plate with chloramphenicol; untransformed cells

  • Plate with chloramphenicol; transformed cells

  • Plate with glucose as carbon source; untransformed cells

  • Plate with glucose as carbon source; transformed cells

  • Plate with sucrose as carbon source; untransformed cells

  • Plate with sucrose as carbon source; transformed cells

  • Control plate with no sugars; untransformed cells

  • Control plate with no sugars; transformed cells


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