Part:BBa_K914009:Experience

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The leakiness of the Kan* gene is higher than expected, and thus one mutation is not sufficient for the containment. Indeed, at usual concentration, Kan* gene manages to express an antibiotic resistance, even though lower than Kan^R or supD (Figure 4A). Here we define the growth rate as the OD₆₀₀ observed at a given time (here t=8h20'), in order to overcome the fact that Kan*+RFP does not have a clear exponential phase. The fact that the strain (3) grows faster and can have higher OD₆₀₀ may be explain by the fact that supD is deleterious for the strain, by removing some stop codon of other genes for instance.

Figure 4A and 4B: The bar graph represent the OD₆₀₀ at time = 8h20' (black line Figure 4C) for the different strains at a given kanamycin concentration (black line on Figure 4B). On B, we observe the variations of the OD₆₀₀ at different kanamycin concentrations, at the same time.

Figure 4C: The variations of the OD₆₀₀ is observed in function of the time, for different concentrations of kanamycin (400µg/mL, 100µg/mL, 25µg/mL).

This part has been further characterised and improved by Edinburgh UG 2018:

http://2018.igem.org/Team:Edinburgh_UG/Results

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