Composite
Part:BBa_K912029:Design
Designed by: Grant Nicholas Group: iGEM12_Northwestern (2012-10-03)
Pgad/lysis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2609
Illegal NheI site found at 2632 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2214
Illegal AgeI site found at 2284 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2865
Design Notes
None besides primer design
Source
Pgad: PCR from vector from CUHK 2011 team Lysis: registry