Composite

Part:BBa_K912029:Design

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-03)


Pgad/lysis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2609
    Illegal NheI site found at 2632
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2214
    Illegal AgeI site found at 2284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2865


Design Notes

None besides primer design


Source

Pgad: PCR from vector from CUHK 2011 team Lysis: registry

References