Composite

Part:BBa_K912015:Design

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-01)


BBa_J23114(weak) Constitutive promoter + ClC-ec1 chloride antiporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
    Illegal AgeI site found at 1323
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None besides primer design for pcring the CLC-ec1


Source

Constitutive Promoter: registry CLC-ec1: genomic sequence

References