Composite

Part:BBa_K912006:Design

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-09-28)


Intracellular Chloride Ion Inducible Lysis Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2609
    Illegal NheI site found at 2632
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2214
    Illegal AgeI site found at 2284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2865


Design Notes

Pgad did not come from the registry. The CUHK team sent the part in an unknown vector and the Pgad promoter was PCR'd out.


Source

Pgad: CUHK 2011 iGEM team Lysis: parts registry

References