Regulatory

Part:BBa_K909012:Design

Designed by: Deborah Huber   Group: iGEM12_ETH_Zurich   (2012-09-23)

PR promoter with multiple operator sites: cI OR1, cI OR2 and and LacI O1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PR region is kept conserved to prevent performance loss. The lacO1 is kept downstream of the operator similar to the natural lac operon.

Source

The design was adapted from research of Kinkhabwala and Guet, 2008.

References

[http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0002030 Kinkhabwala, A., & Guet, C. C. (2008) "Uncovering cis regulatory codes using synthetic promoter shuffling."]