Composite

Part:BBa_K908016:Design

Designed by: Wacquier Benjamin   Group: iGEM12_ULB-Brussels   (2012-09-19)

Microcin B17, Gene B flanked by Attc sequence and rbs


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is preceded and followed by restriction sequences:

For: 5'-GCAGAATTCGCGGCCGCTTCTAGAGCCTCAGCTACACGTGCACTG-3'
Rev: 5'-AGCCTGCAGCGGCCGCTACTAGTA-3'


This parts were synthetised by PCR from plasmid pCID909. The primers were designed in order to add rbs, attc sequence, and RFC10 restriction area.

  • More details here.


    • Source

    BBa_B0030, BBa_J99001 and pCID909

    References