Translational_Unit

Part:BBa_K906103:Design

Designed by: Lucas B. Harrington   Group: iGEM12_WashU   (2012-08-23)

Safranal and Crocin Producing Construct (Cs42s)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 974
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2828
    Illegal SapI site found at 3010


Design Notes

This part has been designed to function in Synechocystis through codon optimization and promoter choice. It also contains restriction sites in between the ORFs as seen in the diagram on the main page. This part has been designed to be as modular as possible, as the individual genes can be easily cut or PCR'd out of the main construct and then used individually.

Source

This part has been designed by using the amino acid sequences from Crocus Sativus and Arabadopsis thalina. Please look at the individual parts pages for more information.

References