Translational_Unit

Part:BBa_K906100

Designed by: Lucas B. Harrington   Group: iGEM12_WashU   (2012-08-13)

Extended psbA2 with Zeaxanthin 7,8-cleavage dioxygenase (ZCD)

This translational unit is designed to constitutivly express Zeaxanthin 7,8-cleavage dioxygenase (ZCD) in Synechocystis. PsbA2 can be removed from this part and utilized with an alternative part by using NdeI to cut between the end of psbA2 and the start codon of ZCD. The ORF to be attached to this promoter should be made to have the same restriction enzyme site (possibly added by PCR) at its 5' end. This will allow for spacing between the regulatory features of psbA2 and the ORF to parallel that of the native use of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 974
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None