Coding

Part:BBa_K906008

Designed by: Lucas B. Harrington   Group: iGEM12_WashU   (2012-08-23)

CrtZ from Arabadobsis

This part codes for the protein sequence of beta-carotene hydroxylase (CrtZ), which converts beta-carotene into zeaxanthin. It has been predicted to form insoluble inclusion bodies (~90% chance) when overexpressed in E. coli according to these two online solubility models: http://mips.helmholtz-muenchen.de/prosoII/prosoII.seam and http://www.biotech.ou.edu/. The source for the coding sequence was found here http://www.ncbi.nlm.nih.gov/nuccore/9230269?from=70&to=1002&report=fasta

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 59
    Illegal SapI site found at 241


[edit]
Categories
Parameters
None