Coding

Part:BBa_K906000:Design

Designed by: Lucas B. Harrington   Group: iGEM12_WashU   (2012-07-30)

Zeaxanthin 7,8-cleavage dioxygenase (ZCD)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 774
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This modified version of ZCD has been codon optimized for the species Synechocystis 6803. It is simply the coding region of the gene without any promoter or terminator, to allow for the most flexible application of this part.

Source

This enzyme was first identified in Crocus sativus, the saffron plant. The originally identified gene can be found here [http://www.ncbi.nlm.nih.gov/nuccore/AJ489276.1 AJ489276.1]

References