Generator

Part:BBa_K902081:Design

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-25)

4S Desulfurization Pathway Optimization Circuit (PlacI/hpaC/RBS/katGLAA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 993
    Illegal BglII site found at 1928
    Illegal BglII site found at 2055
    Illegal BamHI site found at 1845
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 509


Design Notes

None


Source

Built with biobrick parts using basic biobrick assemly method.

References

M A Prieto, E Díaz, and J L García. Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster.J Bacteriol. 1996 January; 178(1): 111–120.