Composite

Part:BBa_K902078:Design

Designed by: Kevin Huie   Group: iGEM12_Calgary   (2012-10-02)

mntP promoter-mntP riboswitch-s7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 327


Design Notes

This circuit was constructed from basic parts using the BioBricks assembly method. The S7 is toxic to cells and therefore these need to be grown up in media that does not contain any manganese.

Source

S7 was obtained from S. aureus. This gene was synthesized from IDT.

References

Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.