Generator

Part:BBa_K902029:Design

Designed by: Himika Dastidar   Group: iGEM12_Calgary   (2012-10-02)

J04500-CviAII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 354
    Illegal BglII site found at 610
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Chlorella virus PCBVI. Part was commercially synthesized. The lacI promoter and ribosome binding site are from the parts registry. This brick was constructed using the basic biobrick assembly method.


References

Xia Y, Burbank DE, Van Etten JL. Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga. Nucl. Acids Res. (1986) 14 (15): 6017-6030.