Generator

Part:BBa_K902029

Designed by: Himika Dastidar   Group: iGEM12_Calgary   (2012-10-02)

J04500-CviAII

The CviaII restriction endonuclease is under the regulation of a low plasmid copy number inducible lacI promoter and a ribosome binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 354
    Illegal BglII site found at 610
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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