Coding

Part:BBa_K900004:Design

Designed by: Austin Jones   Group: iGEM12_Berkeley   (2012-10-02)

Actin Targeting Protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1432
    Illegal PstI site found at 311
    Illegal PstI site found at 1544
    Illegal PstI site found at 1634
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 311
    Illegal PstI site found at 1544
    Illegal PstI site found at 1634
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 33
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1432
    Illegal PstI site found at 311
    Illegal PstI site found at 1544
    Illegal PstI site found at 1634
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1432
    Illegal PstI site found at 311
    Illegal PstI site found at 1544
    Illegal PstI site found at 1634
    Illegal AgeI site found at 142
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1741
    Illegal SapI.rc site found at 1393


Design Notes

The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with our Golden Gate cloning scheme.


Source

Isolated from the yeast genome through PCR.

References