Composite

Part:BBa_K899012:Design

Designed by: Andreas Kjr   Group: iGEM12_SDU-Denmark   (2012-09-26)

SST (BBa_K899001) with double RBS (BBa_K899010)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1845
    Illegal BamHI site found at 138
    Illegal BamHI site found at 628
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 89
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The SST gene derives from the jerusalem artichoke (Helianthus Tuberosus)

References

A mini exon in the sucrose:sucrose 1-fructosyltransferase gene of wheat; Midori Yoshida, Dingbo Lin1, Akira Kawakami [http://www.sciencedirect.com/science/article/pii/S0176161704000975]


Cloning of the fructan biosynthesis pathway of Jerusalem artichoke; Ingrid M. van der Meer, Andries J. Koops, Johanna C. Hakkert and Arjen J. van Tunen[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1998.00230.x/pdf]


Donor and acceptor substrate selectivity among plant glycoside hydrolase family 32 enzymes; Wim Van den Ende, Willem Lammens, Andre´ Van Laere, Lindsey Schroeven and Katrien Le Roy [http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2009.07316.x/pdf]