Composite

Part:BBa_K899011:Design

Designed by: Andreas Kjr   Group: iGEM12_SDU-Denmark   (2012-09-26)

FFT (BBa_K899001) with double RBS (BBa_K899009)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 388
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The FFT gene derives from the jerusalem artichoke (Helianthus Tuberosus)

References

Properties of Fructan:Fructan 1-Fructosyltransferases from Chicory and Globe Thistle, Two Asteracean Plants Storing Greatly Different Types of Inulin Rudy Vergauwen, Andre´ Van Laere, and Wim Van den Ende [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC196615/pdf/1330391.pdf]


Cloning of the fructan biosynthesis pathway of Jerusalem artichoke; Ingrid M. van der Meer, Andries J. Koops, Johanna C. Hakkert and Arjen J. van Tunen[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1998.00230.x/pdf]


Donor and acceptor substrate selectivity among plant glycoside hydrolase family 32 enzymes; Wim Van den Ende, Willem Lammens, Andre´ Van Laere, Lindsey Schroeven and Katrien Le Roy [http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2009.07316.x/pdf]