Part:BBa_K899001:Design

Fructan fructan fructosyltransferase (FFT)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 515
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 369
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

7 BP from start codon we embedded an shine dalgarno sequence.

Source

Extracted from Jesrusalem Artichoke (helianthus tuberosus)

References

Properties of Fructan:Fructan 1-Fructosyltransferases from Chicory and Globe Thistle, Two Asteracean Plants Storing Greatly Different Types of Inulin Rudy Vergauwen, Andre´ Van Laere, and Wim Van den Ende [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC196615/pdf/1330391.pdf]

Cloning of the fructan biosynthesis pathway of Jerusalem artichoke; Ingrid M. van der Meer, Andries J. Koops, Johanna C. Hakkert and Arjen J. van Tunen[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1998.00230.x/pdf]

Donor and acceptor substrate selectivity among plant glycoside hydrolase family 32 enzymes; Wim Van den Ende, Willem Lammens, Andre´ Van Laere, Lindsey Schroeven and Katrien Le Roy [http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2009.07316.x/pdf]