Coding

Part:BBa_K899000:Design

Designed by: Andreas Kjr   Group: iGEM12_SDU-Denmark   (2012-09-22)

Sucrose sucrose fructosyltransferase (SST)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1826
    Illegal BamHI site found at 119
    Illegal BamHI site found at 609
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed illigal restriction sites; we added a Shinedalgarno sequence 7 bp from the coding part, to insure expression, while keeping the Kozak sequence to ensure that the part can still be expressed in plants.


Source

Extracted from Jerusalem artichoke (Helianthus tuberosus).

References

A mini exon in the sucrose:sucrose 1-fructosyltransferase gene of wheat; Midori Yoshida, Dingbo Lin1, Akira Kawakami [http://www.sciencedirect.com/science/article/pii/S0176161704000975]


Cloning of the fructan biosynthesis pathway of Jerusalem artichoke; Ingrid M. van der Meer, Andries J. Koops, Johanna C. Hakkert and Arjen J. van Tunen[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1998.00230.x/pdf]


Donor and acceptor substrate selectivity among plant glycoside hydrolase family 32 enzymes; Wim Van den Ende, Willem Lammens, Andre´ Van Laere, Lindsey Schroeven and Katrien Le Roy [http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2009.07316.x/pdf]