Coding

Part:BBa_K896936:Design

Designed by: Hsun-I Chiu   Group: iGEM12_NYMU-Taipei   (2012-04-25)

Human haptoglobin gene recombinent on Ecoli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 972


Design Notes

Primers used in the PCR: 

Hp_E_FP: gcttctag atgagtgccctgggag (24nt,16nt,TM:54C,%GC:63%)
Hp_E_RP: gct ctgcagcggccgctactagta ttagttctcagctatggtcttctg (48nt,24nt,TM:55C,%GC:42%)

Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.

Source

PCR'd from Escherichia coli str. K-12 substr. MG1655 wildtype phage.

References