![](https://parts.igem.org/images/partbypart/icon_coding.png)
Part:BBa_K895005:Design
Salty_VgrG-CD27_endolysin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 474
Illegal BglII site found at 1806 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1048
Illegal AgeI site found at 1195 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
The vgrG section is derived from BBa_K895002, which was used as a PCR template, and the endolysin section is derived from a synthetic gene designed by Neil Fairweather's group at Imperial College London.
References
The Salmonella Type VI Secretion System is reviewed by these guys:
Blondel,C.J., Jimenez,C.J., Contreras,I. and Santiviago,C.A. (2009) Comparative genomic analysis uncovers three novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics 10:354.
The bacteriophage CD27 endolysin against C. difficile is described here:
Mayer,M.J., Narbad,A. and Gasson,M.J. (2008) Molecular characterization of a Clostridium difficile bacteriophage and its cloned biologically active endolysin. J. Bacteriol. 190:6734-6740.