![](https://parts.igem.org/images/partbypart/icon_coding.png)
Part:BBa_K895004:Design
Salty_Hcp-CD27_endolysin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 306
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 133
Illegal BsaI site found at 150
Illegal SapI site found at 218
Design Notes
None in this case.
Source
The hcp section is derived from BBa_K895001, which was used as a PCR template, and the endolysin section is derived from a synthetic gene designed by Neil Fairweather's group at Imperial College London.
References
The Salmonella Type VI Secretion System is reviewed by these guys:
Blondel,C.J., Jimenez,C.J., Contreras,I. and Santiviago,C.A. (2009) Comparative genomic analysis uncovers three novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics 10:354.
The CD27 endolysin against C. difficile was described here:
Mayer,M.J., Narbad,A. and Gasson,M.J. (2008) Molecular characterization of a Clostridium difficile bacteriophage and its cloned biologically active endolysin. J. Bacteriol. 190:6734-6740.