Coding

Part:BBa_K895004:Design

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-22)

Salty_Hcp-CD27_endolysin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 133
    Illegal BsaI site found at 150
    Illegal SapI site found at 218


Design Notes

None in this case.


Source

The hcp section is derived from BBa_K895001, which was used as a PCR template, and the endolysin section is derived from a synthetic gene designed by Neil Fairweather's group at Imperial College London.

References

The Salmonella Type VI Secretion System is reviewed by these guys:

Blondel,C.J., Jimenez,C.J., Contreras,I. and Santiviago,C.A. (2009) Comparative genomic analysis uncovers three novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics 10:354.

The CD27 endolysin against C. difficile was described here:

Mayer,M.J., Narbad,A. and Gasson,M.J. (2008) Molecular characterization of a Clostridium difficile bacteriophage and its cloned biologically active endolysin. J. Bacteriol. 190:6734-6740.